Highly efficient transformation system for Malassezia furfur and Malassezia pachydermatis using Agrobacterium tumefaciens-mediated transformation
نویسندگان
چکیده
منابع مشابه
Agrobacterium tumefaciens-mediated sorghum transformation using a mannose selection system.
A dual-marker plasmid containing the selectable marker gene, manA, and the reporter gene, sgfp, was used to transform immature sorghum embryos by employing an Agrobacterium-mediated system. Both genes were under the control of the ubi1 promoter in a binary vector pPZP201. The Escherichia coli phosphomannose isomerase (PMI) gene, pmi, was used as the selectable marker gene and mannose was used a...
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An in-house prepared M. furfur antigen was used to carry out a seroprevalence study in an urban population in Malaysia by indirect immunofluorescence assay. Of the 800 serum samples from all ages screened, 738 samples were positive for M. furfur specific IgG, giving an overall seropositive rate of 92.3%. There was no significant difference in the seropositive rates among the different gender gr...
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Agrobacterium tumefaciens transfers a piece of its Ti plasmid DNA (transferred DNA or T-DNA) into plant cells during crown gall tumorigenesis. A. tumefaciens can transfer its T-DNA to a wide variety of hosts, including both dicotyledonous and monocotyledonous plants. We show that the host range of A. tumefaciens can be extended to include Saccharomyces cerevisiae. Additionally, we demonstrate t...
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Agrobacterium tumefaciens is a Gram-negative soil bacterium that causes plant tumours by transferring a portion of DNA from a resident ‘tumour inducing’ (Ti) plasmid into plant cells where it is integrated into a plant chromosome and expressed. This bacteria’s capacity for DNA transfer is the basis of most current plant genetic engineering and make it a model system for the study of pathogenic ...
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Agrobacterium tumefaciens is known to transfer parts of its tumor-inducing plasmid to filamentous fungi including Agaricus bisporus. We have used this transformation system to transform germinating basidiospores of A. bisporus. Analysis of hygromycin B-resistant clones showed that the T-DNA integrated at random sites and in multiple copies into the host genome. Furthermore we have been able to ...
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ژورنال
عنوان ژورنال: Journal of Microbiological Methods
سال: 2017
ISSN: 0167-7012
DOI: 10.1016/j.mimet.2017.01.001